Flow cytometry and cell sorting at the Ruhr University Bochum (RUB)
The core facility for Flow Cytometry and Cell Sorting (FlowCore@RUB) is part of the Medical Research Services of Ruhr University Bochum. It is a core facility of the Faculty of Medicine and is funded by the faculty.
Flow cytometry, or FACS (fluorescence-activated cell sorting), is a key method in biomedical research, as it enables the analysis and isolation of specific cell populations based on various parameters such as size, morphology, and fluorescence intensity. Classical analytical instruments can measure approximately 20 parameters per cell simultaneously, while modern spectral-based systems are capable of resolving up to 40–50 parameters at the single-cell level. This allows high-resolution investigation of cell-type subpopulations and cellular activation states.
Analytical flow cytometry
From intuitive entry-level systems for reliable routine analyses to high-performance instruments for more complex applications, and on to state-of-the-art spectral cytometry for maximum information density – our instruments enable precise cell analysis at every level of expertise.
Cell Sorting
Our high-speed cell sorter MoFlo Astrios EQ enables reliable, high-purity sorting—from complex cell mixtures to single cells. Equipped with multiple high-performance lasers, flexible collection formats, and safe integration within an S2 environment, the system provides optimal conditions for demanding research and clinical projects.
Read More About Cell Sorting
Sample Preparation
The gentleMACS Octo Dissociator enables fully automated processing of multiple samples simultaneously—safe, sterile, and highly reproducible. Thanks to predefined programs, individual heating modules, and customizable protocols, single-cell suspensions or homogenates can be generated efficiently and gently, making the system ideal for downstream analyses and in vitro experiments.
Terms of use
The Core Facility is open to all members of the Ruhr University of Bochum as well as external users, with priority given to the Faculty of Medicine. Clearly defined access regulations, mandatory training, and strict S2 safety standards ensure smooth and safe operations. Additionally, a structured data backup system provides reliable protection for your measurement data.
