Cell sorting

The Core Facility has a high-speed cell sorter that was funded through a major equipment grant from the German Research Foundation (DFG). This device has been in use at the Center for Clinical Research since early 2019. The high-speed cell sorter is a MoFlo Astrios EQ from Beckman Coulter. The sorter is used exclusively by the facility’s staff.

Existing instrument configuration

• 405-nm laser (violet) for excitation of common markers such as DAPI, Cascade Blue, or AmCyan
• 488-nm laser (blue) for versatile standard fluorochromes such as FITC, PE, and PerCP-based dyes
• 561-nm laser (yellow) for highly efficient excitation of PE and red fluorochromes (e.g., Tomato, DsRed)
• 633-nm laser (red) for widely used markers such as APC and Alexa Fluor 700

Our range of sorting options

• Up to six sorting fractions from a single sample
• Single-cell and bulk sorting into tubes, plates, or onto slides
• Up to 40,000 cells per second under biosafety level 2 (BSL-2) containment